Public Resource Page

49 CFR Part 40

The federal rule governing DOT drug and alcohol testing procedures.

The Legal Text Behind Drug & Alcohol Testing

49 CFR Part 40 is the actual federal rule that governs how DOT drug and alcohol testing has to be performed — collection procedures, lab standards, who's qualified to collect and review results, and the return-to-duty process after a violation. It applies across every DOT agency, not just FMCSA, so trucking-specific triggers (who must be tested, and when) come from Part 382 layered on top of this.

Legal Text vs. Plain-Language Guide

Part 40 itself is written as binding regulatory text, not a how-to guide. If you want the plain-language version first, start with the driver-facing brochure below, then come back to Part 40 for the exact legal requirements.

Free Resources

49 CFR Part 40 — Procedures for Transportation Workplace Drug and Alcohol Testing Programs eCFR
The full binding federal rule governing DOT drug and alcohol testing collection, lab, and review procedures.
Drug and Alcohol Testing Brochure for Drivers FMCSA
The plain-language driver-facing explanation of what Part 40 requires in practice.